ZHAO Qiong

Education

2006.09-2012.07   Ph.D.    Peking University (PKU),             Beijing/CHINA

2002.09-2006.07   B.Sc.     East China Normal University (ECNU), Shanghai/CHINA

Qiong ZHAO

Area of Interests and Research Fields

 The eukaryotic endomembrane system (ES) is found only in all eukaryotes, but not in any prokaryotes, which remarks its evolutionary and functional importance to eukaryotic cells.  It separates cells into different compartments known as membrane bound organelles, bridges communications in between organelles via vesicle mediated transports, and is served by hundreds of dedicated proteins.

 Currently, research including experimental study with model eukaryotic cells and phylogenetic study with comparative genomic approach have discovered a set of conserved-core-molecular-machineries (CCMM) orchestrating the basic ES-related cellular events, and the CCMM seems as ancient traits inherited from the last eukaryotic common ancestor (LECA).  

 Modern eukaryotes, as descendants from the LECA, exhibit enormous distinctions in the ES. Plant cell varies in several fundamental factors from other eukaryotic organisms. For instance, plant cells have unique cellular structures like cell wall, chloroplast, central vacuole, and plasmodesmata, and the biogenesis and dynamics of these structures are highly related with the ES. It is of great urgent to characterize plant unique ES-related regulatory paradigm.

  Our lab focuses on the evolution and molecular regulation mechanism of plant cell ES, especially the vacuole-related pathways in the context of plants development and hormone signalling pathways, mainly we use Arabidopsis thaliana and certain types of bryophyte to address above questions.

Education

 2006.09-2012.07, Ph.D.  Peking University (PKU),    Beijing/CHINA

 2002.09-2006.07, B.Sc.   East China Normal University (ECNU), Shanghai/CHINA

Academic Positions

 2020.07-Present    Professor/PI, East China Normal University (ECNU)

 2016.11-2019.04    Research Associate, The Chinese University of Hong Kong (CUHK)

 2012.10-2016.10    Postdoc Research, The Chinese University of Hong Kong (CUHK)

Area of Interests and Research Fields

The eukaryotic endomembrane system (ES) is found only in eukaryotes and not in prokaryotes, highlighting its evolutionary and functional importance to eukaryotic cells. The ES separates cells into membrane-bound organelles, facilitates communication between organelles through vesicle-mediated transport, and relies on numerous dedicated proteins.

Recent research, combining experimental studies with model eukaryotic cells and phylogenetic studies using a comparative genomic approach, has identified a set of conserved core molecular machineries (CCMM) that orchestrate basic ES-related cellular events. These CCMM appear to be ancient traits inherited from the last eukaryotic common ancestor (LECA).

Modern eukaryotes, descendants of LECA, exhibit significant variations in the ES. Plant cells, in particular, differ in several fundamental aspects from other eukaryotic organisms. For example, plant cells possess unique cellular structures such as the cell wall, chloroplasts, central vacuole, and plasmodesmata. The biogenesis and dynamics of these structures are closely linked to the ES. Therefore, it is crucial to characterize the plant-specific ES-related regulatory paradigm.

Our laboratory focuses on studying the evolution and molecular regulatory mechanisms of the plant cell ES, with special emphasis on vacuole-related pathways in the context of plant development and hormone signaling pathways. We primarily use Arabidopsis, certain types of bryophytes, tobaccco and tomato to investigate the aforementioned questions.

发表论文（^#共同第一作者; *通讯作者; h-index: 18; 总引数：2185）

1.      Su, Q., Liu, L., Hu, Z., Wang, T., Wang, H., Guo, Q., Liao, X., Dong, Z.* , Yang, S.* , Liu, N.* , Zhao, Q.* (2023)  Unbiased Estimation of Chloroplast Number in Plant Cells Using Deep Learning Methods. bioRxiv 2023.12.17.572064; doi:https: //doi.org/10.1101/2023.12.17.572064 

2.       Zhu, Y., Zhao, Q., Cao, W., Huang, S., Ji, C., Zhang, W., Trujillo, M., ShenJ.,* and Jiang L., *(2023) The plant-unique protein DRIF1 coordinates with SNX1 to regulate membrane protein homeostasis. Plant Cell  . https://doi.org/10.1093/plcell/koad227.（Journal metrics:Impact Score 6.75, H-Index 341, 5-year IF 12.06）

3.       Zeng, Y^#., Liang, Z^#., Liu, Z^#., Li, B^#., Cui, Y., Gao, C., Shen, J., Wang, X., Zhao, Q., Zhuang, X., Erdmann, P.S., Wong, K.-B. and Jiang, L. *(2023). Recent advances in plant endomembrane research and new microscopical techniques. New Phytologist. https://doi.org/10.1111/nph.19134（Journal metrics: Impact Score 8.75, H-Index 285, 5-year IF 8.08）

4.       Hassani, D., Lu, Y., Ni, B., Zhu, R., Zhao, Q.*(2023). The endomembrane system: how does it contribute to plant secondary metabolism? Trends in Plant Science.ISSN 1360-1385 （Journal metrics: Impact Score 11.39, H-Index 263, 5-year IF 20.34） 

5.       Neuhaus, J*, Pimpl, P*, Zhao, Q.* and Wang H.* (2023). Editorial: Regulation of plant organelle biogenesis and trafficking. Frontiers in Plant Science. 14:1211807.（Journal metrics: Impact Score 5.44, H-Index 125, 5-year IF 5.69）

6.       王博之，赵琼*.(2023). 植物内膜系统中的水通道蛋白研究进展. 生物学教学. 48 (05): 2-4.（Journal metrics: 全国教育类核心期刊, 5-year IF 0.457）

7.       Liu, H., Shen, C., Hassani, D., Fang, W., Wang, Z., Lu, Y., Zhu, R., Zhao, Q.* (2022). Vacuoles in bryophytes: properties, biogenesis, and evolution. Frontiers in Plant Science. 13，863389.（Journal metrics: Impact Score 5.44, H-Index 125, 5-year IF 5.69）

8.       Huang, S., Liu, Z., Cao, W., Li, H., Zhang, W., Cui, Y., Hu, S., Luo, M., Zhu, Y., Zhao, Q., Xie, L., Gao, C., Xiao, S., Jiang, L.*(2022). The plant ESCRT component FREE1 regulates peroxisome-mediated turnover of lipid droplets in germinating Arabidopsis seedlings.Plant Cell 34(11):4255-4273（Journal metrics:Impact Score 6.75, H-Index 341, 5-year IF 12.06）

9.       Cui, Y.^#,*, Zhao, Q.^#, Hu, S.,and Jiang, L^*. (2020). How many models and how many vacuoles?Trends in Plant Science 25 (6): 538-548.（Journal metrics: Impact Score 11.39, H-Index 263, 5-year IF 20.34）

10.       Zhu, Y.^#, Ji, C.^#, Cao, W., Shen, J., Zhao, Q., Jiang, L.* (2020). Identification and characterization of unconventional membrane protein trafficking regulators in Arabidopsis: A genetic approach. Journal of Plant Physiology 252, 153229 （Journal metrics:Impact Score 3.45, H-Index 130, 5-year IF 3.54）

11.    Zhao, Q.^#,*,Shen, J.^#, Gao, C.^#, Cui, Y.^#, Wang, Y., Cui, J.,Cheng, L.,Cao, W., Zhu, Y., Huang, S., Zhou, Q., Leong, C., Leung, K., Chen, X., and Jiang, L.^*(2019). RST1 is a FREE1 suppressor that negatively regulates vacuolar trafficking in Arabidopsis. Plant Cell 31, 2152-2168.（Journal metrics:Impact Score 6.75, H-Index 341, 5-year IF 12.06）

12.    Zhao, Q. ^*, Zhu, Y., Cao, W., Shen, J., Cui, Y., Jiang, L. (2019). Genetic suppressor screen using an inducible FREE1-RNAi line to detect ESCRT genetic interactors in Arabidopsis thaliana. Methods in Molecular Biology 1998:273-289 (Book chapter)

13.    Li, H.^#, Li Y.^#, Zhao, Q.^#, Li, T., Wei, J., Li B., Shen, W., Yang, C., Zeng, Y., Rodriguez, P., Zhao, Y., Jiang, L.^*, Wang, X.^*, and Gao, C.^*(2019) The Plant ESCRT Component FREE1 Shuttles to the Nucleus to Attenuate Abscisic Acid Signaling. Nature Plants 5, 512–524. （Journal metrics:Impact Score 7.11, H-Index 71, 5-year IF 7.22）

14.    Cui, Y.^#,*, Cao, W.^#, He, Y.^#, Zhao, Q., Wakazaki, M., Zhuang, X., Gao, J., Zeng, Y., Gao, C., Ding, Y., Wong, H.Y., Wong, W.S., Lam, H.K., Wang, P., Ueda, T., Rojas-Pierce, M., Toyooka, K., Kang, B.H., and Jiang, L.^*(2019). A whole-cell electron tomography model of vacuole biogenesis in Arabidopsis root cells. Nature Plants 5, 95-105. （Journal metrics:Impact Score 7.11, H-Index 71, 5-year IF 7.22）

15.    Wang, H.^#, Zhao, Q.^#, Fu, J., Wang, X., and Jiang, L.^*(2018). Re-assessment of biolistic transient expression: An efficient and robust method for protein localization studies in seedling-lethal mutant and juvenile plants. Plant Science 274, 2-7. (Cover Story) （Journal metrics:Impact Score 4.54, H-Index 150, 5-year IF 4.253）

16.    Shen, J.^#,*, Zhao, Q.^#, Wang, X., Gao, C., Zhu, Y., Zeng, Y., and Jiang, L.^*(2018). A plant Bro1 domain protein BRAF regulates multivesicular body biogenesis and membrane protein homeostasis. Nature Communication 9, 3784. （Journal metrics:Impact Score 13.78, H-Index 365, 5-year IF 14.12）

17.    Cui, Y.^#, Zhao, Q.^#, Xie, H.T., Wong, W.S., Wang, X., Gao, C., Ding, Y., Tan, Y., Ueda, T., Zhang, Y., and Jiang, L.^*(2017). MONENSIN SENSITIVITY1 (MON1)/CALCIUM CAFFEINE ZINC SENSITIVITY1 (CCZ1)-Mediated Rab7 Activation Regulates Tapetal Programmed Cell Death and Pollen Development. Plant Physiology 173, 206-218. （Journal metrics:Impact Score 6.79, H-Index 312, 5-year IF 7.52）

18.    Shen, J.^#, Gao, C.^#, Zhao, Q., Lin, Y., Wang, X., Zhuang, X., and Jiang, L.^*(2016). AtBRO1 Functions in ESCRT-I Complex to Regulate Multivesicular Body Protein Sorting. Molecular Plant 9, 760-763. （Journal metrics:Impact Score 9.6, H-Index 115, 5-year IF 9.91）

19.    Belda-Palazon, B., Rodriguez, L., Fernandez, M.A., Castillo, M.C., Anderson, E.M., Gao, C., Gonzalez-Guzman, M., Peirats-Llobet, M., Zhao, Q., De Winne, N., Gevaert, K., De Jaeger, G., Jiang, L., Leon, J., Mullen, R.T., and Rodriguez, P.L.^*(2016). FYVE1/FREE1 Interacts with the PYL4 ABA Receptor and Mediates Its Delivery to the Vacuolar Degradation Pathway. Plant Cell 28, 2291-2311.（Journal metrics:Impact Score 6.75, H-Index 341, 5-year IF 12.06）

20.     Cui, Y., Gao, C., Zhao, Q., Jiang, L.^*(2016). Using Fluorescent Protein Fusions to Study Protein Subcellular Localization and Dynamics in Plant Cells. Methods in Molecular Biology 1474:113-123. (Book chapter)

21.    Zhao, Q., Gao, C., Lee, P., Liu, L., Li, S., Hu, T., Shen, J., Pan, S., Ye, H., Chen, Y., Cao, W., Cui, Y., Zeng, P., Yu, S., Gao, Y., Chen, L., Mo, B., Liu, X., Xiao, S., Zhao, Y., Zhong, S., Chen, X., and Jiang, L.^*(2015). Fast-suppressor screening for new components in protein trafficking, organelle biogenesis and silencing pathway in Arabidopsis thaliana using DEX-inducible FREE1-RNAi plants. Journal of Genetics and Genomics 42, 319-330. (Cover Story) （Journal metrics:Impact Score 2.26, H-Index 58, 5-year IF 2.62

22.    Zhang, X., Zhu, Y., Liu, X., Hong, X., Xu, Y., Zhu, P., Shen, Y., Wu, H., Ji, Y., Wen, X., Zhang, C., Zhao, Q., Wang, Y., Lu, J., and Guo, H.^*(2015). Plant biology. Suppression of endogenous gene silencing by bidirectional cytoplasmic RNA decay in Arabidopsis.Science 348, 120-123. （Journal metrics:Impact Score 13.08, H-Index 1186, 5-year IF 44.37）

23.    Gao, C.^#, Zhuang, X^#., Cui, Y., Fu, X., He, Y., Zhao, Q., Zeng, Y., Shen, J., Luo, M., and Jiang, L.^*(2015). Dual roles of an Arabidopsis ESCRT component FREE1 in regulating vacuolar protein transport and autophagic degradation. Proc Natl Acad Sci USA 112, 1886-1891.（Journal metrics:Impact Score 9.57, H-Index 771, 5-year IF 9.83）

24.    Gao, C., Zhao, Q., and Jiang, L.^*(2015). Vacuoles protect plants from high magnesium stress. Proc Natl Acad Sci USA 112, 2931-2932. （Journal metrics:Impact Score 9.57, H-Index 771, 5-year IF 9.83） 

25.    Gao, C., Luo, M., Zhao, Q., Yang, R., Cui, Y., Zeng, Y., Xia, J., and Jiang, L.^*(2014). A unique plant ESCRT component, FREE1, regulates multivesicular body protein sorting and plant growth. Current Biology 24, 2556-2563. （Journal metrics:Impact Score 5.99, H-Index 316, 5-year IF 6.22）

26.    Cui, Y., Zhao, Q., Gao, C., Ding, Y., Zeng, Y., Ueda, T., Nakano, A., and Jiang, L.^*(2014). Activation of the Rab7 GTPase by the MON1-CCZ1 Complex Is Essential for PVC-to-Vacuole Trafficking and Plant Growth in Arabidopsis. Plant Cell 26, 2080-2097. （Journal metrics:Impact Score 6.75, H-Index 341, 5-year IF 12.06）

27.    Wen, X.^#, Zhang, C.^#, Ji, Y.^#, Zhao, Q., He, W., An, F., Jiang, L., and Guo, H.^*(2012). Activation of ethylene signaling is mediated by nuclear translocation of the cleaved EIN2 carboxyl terminus. Cell Research 22, 1613-1616.（Journal metrics:Impact Score 10.53, H-Index 167, 5-year IF 11.43）

28.    Zhao, Q., and Guo, H.^*(2011). Paradigms and Paradox in the Ethylene Signaling Pathway and Interaction Network. Molecular Plant 4, 626-634. （Journal metrics:Impact Score 9.6, H-Index 115, 5-year IF 9.91）

29.    赵琼, 何文容, 张新岩，郭红卫^*. (2010). 拟南芥乙烯信号转导机理的遗传学和化学生物学研究.生命科学 22(11): 1167-1172. （Journal metrics: 北大核心期刊, 2022 IF 1.19）

30.    An, F.^#, Zhao, Q.^#, Ji, Y., Li, W., Jiang, Z., Yu, X., Zhang, C., Han, Y., He, W., Liu, Y., Zhang, S., Ecker, J.R., and Guo, H.^*(2010). Ethylene-Induced Stabilization of ETHYLENE INSENSITIVE3 and EIN3-LIKE1 Is Mediated by Proteasomal Degradation of EIN3 Binding F-Box 1 and 2 That Requires EIN2 in Arabidopsis. Plant Cell 22, 2384-2401. （Journal metrics:Impact Score 6.75, H-Index 341, 5-year IF 12.06）

31.    Zhong, S., Zhao, M., Shi, T., Shi, H., An, F., Zhao, Q., and Guo, H.^*(2009). EIN3/EIL1 cooperate with PIF1 to prevent photo-oxidation and to promote greening of Arabidopsis seedlings. Proc Natl Acad SciUSA 106, 21431-21436. （Journal metrics:Impact Score 9.57, H-Index 771, 5-year IF 9.83）