Helin

01/2023 – 12/2023     Honorary Associate Professor,visiting scholar

                    Macquarie University, Sydney, Australia

01/2013 – now           Associate Professor

                    East China Normal University, Shanghai, China

01/2014 – 01/2015     Visiting Scholar

                    Cornell University, Ithaca, NY, USA

10/2011 – 12/2012     Research Assistant Professor

                    East China Normal University, Shanghai, China

04/2009 – 10/2011     Postdoctoral Research Associate

                    East China Normal University, Shanghai, China

My research experience has been focusing on the male reproduction, especially on the accessory sex gland protein function in sperm activation and sperm membrane protein modification and sperm maturation. We had completed the transcriptome analysis of accessory sex gland and testis, and also the proteomic analysis of sperm, accessory sex gland and testis have been done. We had obtained such data set and want to find more interesting regulation mechanisms in spermatogenesis, sperm maturation, and interaction of spermatozoa and egg. Based on the high-throughput sequencing results and bioinformatics analysis we identified many important signal pathways involved in reproduction, such as MAPK and WNT signal pathways, I have applied four funds from National Natural Science Foundation of China and achieved perfect production in relevant research area. I studied in Cornell University as a visiting scholar for one year in 2014, and cooperation professor is Mariana Wolfner, who is member of the US National Academy of Sciences. She is excellent in reproduction research of Drosophila and I established the CRISPR/Cas9 system in her lab during visiting study and learned the accessory sex gland protein function research system. Male AGPs can also regulate proteolysis, thereby promoting the effective conduct of mating behavior. Serine protease proteins have been proved to play a role in this process in many species. Seminase causes protease to hydrolyze accessory gland protein in the process of starting the protease cascade signal pathway and participates in the early regulation process after mating. Serine protease 2 (Ser2) is also very important for reproduction in silkworm. We knockout the Ser2 gene using CRISPR/Cas9 technology in silkworm, it is found that Ser2 mutation causes sperm can’t enter the egg smoothly, resulting in male mutant sterility. Wild type females and Ser2 mutant males lay eggs normally but do not hatch after sexual mating, and Ser2 mutation does not affect the normal progress of other reproductive behaviors, Moreover, male sterility can be stably passed on to offspring. In addition, similar male sterility phenotypes were obtained by knocking out the Ser2 homologous gene in the lepidopteran pest Plutella xylostella. These results unanimously show that Ser2 gene is an essential factor for male reproductive success. It can be seen that proteases and protease inhibitors are another important protein family of paragonadal proteins that regulate sperm function. 

1.    National Natural Science Foundation of China General Project, Role of serine protease in the regulation of sperm function in Bombyx mori. Project Approval No. 31970476 (2020.01-2023.12, RMB 560,000) CIA

2.    National Natural Science Foundation of China General Project, Function of accessory gland proteins in the mating and reproduction behavior in Bombyx mori. Project Approval No. 31772517 (2018.01-2021.12, RMB 580000) CIA

3.    National Natural Science Foundation of China General Program, Function of ubiquitin proteasome system in sperm acrosome reaction in Chinese mittern crab, Eriocheir sinensis. Project Approval No. 41376157 (2014.01-2017.12, RMB 770,000) CIA

4.    National Natural Science Foundation of China Youth Project, Function of ERKs and p38 in capacitation and acrosome reaction in Chinese mitten crab, Eriocheir sinensis. Project Approval No. 31201974 (2013.01-2015.12, RMB 240,000) CIA

5.    Shanghai Natural Science Fund Committee on Project, Establishment of female follicular development defect model by CRISPR/Cas9 gene editing technology. Project Approval No. 18140902200, (2018.05-2021.04, RMB 300,000) CIA

6.    Shanghai Natural Science Fund Committee on Project, Function of fertilin gene and protein in Chinese mitten crab, Eriocheir sinensis. Project Approval No. 12ZR1408900 (2012.10-2015.08, RMB 100,000) CIA

7.    Doctoral Fund of Ministry of education of the new teacher project, Function of fertilin in Chinese mitten crab, Eriocheir sinensis. Project Approval No.20120076120011 (2013.01-2015.12, RMB 40,000) CIA

Funds

1. National Natural Science Foundation of China General Program, 2014.01-2017.12

2. National Natural Science Foundation of China Youth Program,  2013.01-2015.12

3.Shanghai Natural Science Fund Committee General Program, 2012.10-2015.08

4.Doctoral Fund of Ministry of education of the new teacher program, 2013.01-2015.12

Publications

After 2013 

[28] Jinjin Ding, Chunlai Cui, Guandong Wang, Ge Wei, Liang Bai, Yifei Li, Peilu Sun, Ling Dong, Zicheng Liu, Jiaqi Yun, Fang Li, Kai Li, Lin He, and Sibao Wang. Engineered gut symbiotic bacterium-mediated RNAi for effective control of Anopheles mosquito larvae. Microbiology Spectrum, 2023, 11(4): e01666-23.

[27] Xia Xu, Yao-Hui Wang, Zu-Lian Liu , Yong-Qiang Wang, Lin He, Kai Li, Yong-Ping Huang*. Disruption of egg-specific protein causes female sterility in Bombyx mori.  Insect Science, 2022, 29(1):128-138.

[26] Xu Xia, Wang Yao hui, Bi Hong lun, Xu Jun, Liu Zu lian, Niu Chang Ying, He Lin*, Anthony A. James, Li Kai *, Huang Yong Ping *. Mutation of the seminal protease gene, serine protease 2, results in male sterility in diverse lepidopterans. Insect Biochemistry and Molecular Biology, 2020, 116: 103243. 

[25] Xu Xia, Bi Hong Lun, Wang Yao Hui, Li Xiao Wei, Jun Xu, Liu Zu Lian, Lin He*，Li Kai*，Huang Yong Ping*. Disruption of the ovarian serine protease (Osp) gene causes female sterility in Bombyx mori and Spodoptera litura. Pest Management Science, 2020,76:1245-1255. 

[24] Gao Yun xia, Bu Yun, He Lin*, Li Kai. A new species in the genus Dimorphacanthella (Collembola: Isotomidae) from Sichuan Province, Southwest China. Entomotaxonomia (2020) 42(1): 1–5.

[23] Bi Hong Lun,  Xu Jun, He Lin, Zhang Yong, Li Kai, Huang Yong Ping.CRISPR/Cas9-mediated ebony knockout results in puparium melanism in Spodoptera litura.  Insect Science. 2019 Dec; 26(6): 1011-1019. 

[22] Xu Xia , Zhang Zhong Jie , Yang Yang, Huang Si Rui , Li Kai , He Lin* and  Zhou Xu Guo. Genome editing reveals the function of Yorkie during the embryonic and early larval development in silkworm, Bombyx mori. Insect Molecular Biology, 2018,27(6):675-685.

[21] Yang Yang, Wang Yao Hui , Chen Xi'en, Tian Di, Xu Xia, Li Kai , Huang Yong Ping, He Lin*. CRISPR/Cas9 mediated Tyrosine hydroxylase knockout resulting in larval lethal in Agrotis ipsilon.Insect Science, 2018,25,1017-1024.

[20] Xu Xia, Bi Hong Lun, Zhang Zhong Jie , Yang Yang, Li Kai, Huang Yong Ping, Zhang Yong, He Lin*. BmHpo mutation induces smaller body size and late stage larval lethality in the silkworm, Bombyx mori. Insect Science, 2018,25,1006-1016.

[19] Akanksha Singh, Norene A. Buehner, He Lin, Kaitlyn J. Baranowski, Geoffrey D. Findlay and Mariana F. Wolfner*. Long-term interaction between Drosophila sperm and sex peptide is mediated by other seminal proteins that bind only transiently to sperm. Insect Biochemistry and Molecular Biology. 2018, 102: 43-51.

[18] Qing Li, Hong-Dan Yang, Lin He, Qun Wang. Characterization of the Es-DDX52 involved in the spermatogonial mitosis and spermatid differentiation in Chinese mitten crab (Eriocheir sinensis）.Gene, 2018, 646: 106-119.

[17] Hong-Dan Yang, Qing Li, Ai-Qing Yu, Lin He*, Qun Wang. Molecular characterization and sub-celluiar distribution of JNK and JIP4 protein kinases in spermatogenesis and acrosome reaction of the Chinese mitten crab Eriocheir sinensis. Journal of Crustacean Biology, 00(0),1-11,2016. DOI: 10.1163/1937240X-00002468.

[16] Yuan-Li Wang, Dan Li, Hong-Dan Yang, Lin He, Wen-Juan Sun, Ze-Lin Duan, Qun Wang. The E3 ubiquitin ligase CRL4 regulates proliferation and progression through meiosis in Chinesemitten crab Eriocheir sinensis, Biology of Reproduction. 2016, 94(3), pp 1-14, 2016 3.

[15] Jin Cheng Zhao, Yuan Li Wang, Qing Li, Ming Zhu, Wen Juan Sun, Tian Min Wu, Qun Wang, Lin He*. Molecular Cloning and Characterization of p38 Gene in the Chinese Mitten Crab, Eriocheir sinensis. Aquaculture Research. 2016, 47: 1353-1363.

[14] He L, Wang YL, Li Q, Yang HD, Duan ZL, Wang Q.  Profiling microRNAs in the testis during sexual

 maturation stages in Eriocheir sinensis. Animal Reproduction Science. 2015 Nov. 162: 52-61.  doi:10.1016/j.anireprosci.2015.09.008

[13] Li Q, Xie J, He L, Wang Y, Yang H, Duan Z, Wang Q. FOXL2 Down-regulates Vitellogenin 

Expression at Mature Stage in Eriocheir sinensis. Biosci Rep. 2015 Oct 1. pii: BSR20150151. 
[12] Li Q, Liu L, Wang Y, Xie J, He L*, Wang Q. Characterization and expression analysis of serpins in the 

Chinese mitten crab Eriocheir sinensis. Gene. 2015 Sep 21. pii: S0378-1119(15)01142-7.

doi: 10.1016/j.gene.2015.09.033

[11] He L, Li Q, Liu L, Wang Y, Xie J, Yang H, Wang Q. A Catalog of Proteins Expressed in the AG Secreted Fluid during the Mature Phase of the Chinese Mitten Crabs (Eriocheir sinensis). PLoS One. 2015 Aug 25;10(8):e0136266. doi: 10.1371/journal.pone.0136266. 

[10] Yuanli Wang,Wenjuan Sun, Lin He, Qing Li,Qun Wang.Morphological alterations of all stages of spermatogenesis and acrosome reaction in Chinese mitten crab Eriocheir sinensis. Cell Tissue Res. 2015 May;360(2):401-12.

[9] Qing Li, Yuanli Wang, Jing Xie, Wenjuan Sun, Ming Zhu, Lin He*, Qun Wang^*. Characterization and expression of DDX6 during gametogenesis in the Chinese mitten crab Eriocheir sinensis. Genet. Mol. Res. 2015, 14 (2): 4420-4437.

[8] Qing Li,Jing Xie, Lin He, Yuanli Wang, Zeling Duan, Hongdan Yang, Qun Wang. Identification of ADAM10 and ADAM17 with potential roles in the spermatogenesis of the Chinese mitten crab, Eriocheir sinensis. Gene. 2015 May 10;562(1):117-27.

[7] Sun WJ, Zhu M, Wang YL, Li Q, Yang HD, Duan ZL, He L*,WangQ*. ERK Is Involved in the Process of Acrosome Reaction In Vitro of the Chinese Mitten Crab, Eriocheir sinensis. Mar Biotechnol (NY).2015, 17(3):305-16.

[6] Zhu M, Sun WJ, Wang YL, Li Q, Yang HD, Duan ZL, He L*, Wang Q*. P38 participates in spermatogenesis and acrosome reaction prior to fertilization in Chinese mitten crab Eriocheir sinensis. Gene. 2014 Nov 26. pii: S0378-1119(14)01327-4. doi: 10.1016/j.gene.2014.11.050.

[5] Wang YL, Li Q, Xie J, Zhu M, Sun WJ, He L*, Wang Q*. Involvement of the single Cul4 gene of Chinese mitten crab Eriocheir sinensis in spermatogenesis. Gene. 2014 Feb 15;536(1):9-17. doi: 10.1016/j.gene.2013.11.099.

[4] Lin He, Hui Jiang, Dandan Cao, Lihua Liu, Songnian Hu, and Qun Wang. Comparative Transcriptome Analysis of the Accessory Sex Gland and Testis from the Chinese Mitten Crab (Eriocheir sinensis). PLoS One. 2013, 8: e53915. doi: 10.1371/journal.pone.0053915.

[3] Lin He, Jing Xie, Qing Li, Yunlong Zhao, Yaping Wang and Qun Wang. Isolation and Characterization of Polymorphic Microsatellite Loci in the Redclaw Crayfish, Cherax Quadricarinatus. J Aquac Res Development，2013, 4:162 doi:10.4172/2155-9546.1000162.

[2] Juan Wang, Di-An Fang, Yang Wang, Yuan-Li Wang, Lin Cheng, Lin He, and Qun Wang. Cathepsin A protein from accessory sex gland of the Chinese mitten crab (Eriocheir sinensis) plays a key role in spermatophore digestion. Journal of Insect Physiology.2013,,59 (2013) 953–960. DOI: 10.1016/j.jinsphys.2013.07.0032013.

[1] Lin Cheng, Xing-Kun Jin, Wei-Wei Li , Shuang Li, Xiao-Nv Guo, Juan Wang, Ya-Nan Gong, Lin He, Qun Wang. Fatty acid binding proteins FABP9 and FABP10 participate in antibacterial responses in Chinese mitten crab, Eriocheir sinensis.PLoS ONE 01/2013; 8(1): e54053. dio: 10.1371/journal.pone.0054053.

Before 2012

[1] Lin He, Qun Wang, XingKun Jin, Ying Wang, LiLi Chen, LiHua Liu, Yang Wang. Transcriptome profiling of testis during sexual maturation stages in Eriocheir sinensis using Illumina sequencing. PLoS One. 2012,7(3):e33735. doi:10.1371/journal.pone.0033735.

[2] Lin He, YanNan Xie, Wei Lu, Ying Wang, LiLi Chen, Peter B Mather, YunLong Zhao,YaPing Wang, Qun Wang. Genetic diversity in three redclaw crayfish (Cherax quadricarinatus) lines developed in culture in China. Aquaculture Research. 2012, 43, 75–83. 

[3] Yan nan Xie, Lin He, Jiang ling Sun, Li li Chen, Yun long Zhao, Ya ping Wang and Qun Wang. 2010. Isolation and characterization of fifteen microsatellite loci from the redclaw crayfish, Cherax quadricarinatus. Aquat. Living Resour. 2010，23, 231–234. 

[4] Lin He, Si bao Wang, Xue xia Miao, Hong Wu, Yong ping Huang. Identification of necrophagous fly species using ISSR and SCAR markers, Forensic Science International，2007，168: 148–153. 

[5] Lin He, Qun Wang. Review on the PCR-Based SSR Markers Isolation Methods. Genomics and Applied Biology, 2010,29(4):775-782. (In Chinese)

[6] Lin He, Li Hua Liu, Yang Wang. Cloning and sequence analysis of para sodium channel cDNA fragment from silkworm, Bombyx mori. Chinese Journal of Applied Entomology. 2011, 48(3):596-604. (In Chinese). 

[7] WeiWei Li, Lin He, XingKun Jin, Hui Jiang, LiLi Chen, Ying Wang, Qun Wang. Molecular cloning,characterization and expression analysis of cathepsin A gene in Chinese mitten crab, Eriocheir sinensis. Peptides, 2011,32(3): 518-525.

[8] Xing-Kun Jin, Wei-Wei Li, Lin He, Hui Jiang, Ying Wang, Li-Li Chen, Qun Wang. Molecular cloning, characterization and expression analysis of two apoptosis genes, caspase and nm23, involved in the antibacterial response in Chinese mitten crab, Eriocheir sinensis. Fish and Shellfish Immunology, 2011, 30(1): 263-272.

[9] WeiWei Li, XingKun Jin, Lin He, YaNan Gong, Hui Jiang, Qun Wang. Molecular cloning and tissue expression of the fatty acid-binding protein (Es-FABP9) gene in the reproduction seasons of Chinese mitten crab, Eriocheir sinensis. Molecular Biology Reports, 2010, doi: 10.1007/s11033-010-0666-1.

[10] WeiWei Li, XingKun Jin, Lin He, Hui Jiang, YaNan Gong, YanNan Xie, Qun Wang. Molecular cloning, characterization, expression and activity analysis of cathepsin L in Chinese mitten crab, Eriocheir sinensis. Fish and Shellfish Immunology,2010,29(6): 1010-18.

[11] Xue-Li Hou, Qian Mao,Lin He, Ya-Nan Gong, Di Qu, and Qun Wang. Accessory Sex Gland Proteins Affect Spermatophore Digestion Rate and Spermatozoa Acrosin Activity in Eriocheir sinensis. Journal of Crustacean Biology, 2010, 30(3): 435-440.

[12] Wei-Wei Li, Xing-Kun Jin, Lin He, Hui Jiang, Yan-Nan Xie, Qun Wang (2010) Molecular cloning, characterization and expression analysis of cathepsin C gene involved in the antibacterial response in Chinese mitten crab, Eriocheir sinensis. Developmental and Comparative Immunology, 2010, 34(11):1170-1174.

[13] WeiWei Li, XingKun Jin, Lin He, Ying Wang, LiLi Chen, Hui Jiang, Qun Wang. Molecular cloning, characterization and expression analysis of macrophage migration inhibitory protein (MIF) in Chinese mitten crab, Eriocheir sinensis. Fish and shellfish immunology, 2011, 30(1): 324-329.

[14] DiAn Fang, Qun Wang, Lin He, Juan Wang, Yang Wang. Characterization of heat shock protein 70 in the red claw crayfish (Cherax quadricarinatus): Evidence for its role in regulating spermatogenesis. Gene, 2012‚492（1）：138-147. (2010 IF:2.266)

[15] Hui Jiang, Fei Ren, Jiangling Sun, Lin He, Weiwei Li, Yannan Xie, Qun Wang. Molecular Cloning and Gene Expression Analysis of the Leptin Receptor in the Chinese Mitten Crab Eriocheir sinensis. PLoS ONE, 2010, 5(6): e11175.

[16] Wei-Wei Li, Ya-Nan Gong, Xing-Kun Jin, Lin He, Hui Jiang, Fei Ren, Qun Wang (2010) The effect of dietary zinc supplementation on the growth, hepatopancreas fatty acid composition and gene expression in the Chinese mitten crab, Eriocheir sinensis (H. Milne-Edwards) (Decapoda: Grapsidae). Aquaculture Research. 2010, 41: e828-e837.

[17] Fei Ren, Hui Jiang, Jiangling Sun, Lin He, Weiwei Li, Ying Wang and Qun Wang. Cloning‚ characterization‚ expression‚ and copper sensitivity of the metallothionein-1 gene in the Chinese Mitten Crab‚ Eriocheir sinensis.Molecular Biology Reports‚38(4):2383-93.

[18] Lu Wei, Li WeiWei, Jin XinKun, He Lin, Jiang Hui, Wang Qun. (2011) Reproductive function of Selenoprotein M in Chinese mitten crabs (Eriocheir sinesis). Peptides.

[19] DiAn. Fang, Qun Wang, Juan Wang, Lin He, LiHua Liu and Ying Wang. A novel DDX5 gene in the freshwater crayfish Cherax quadricarinatus is highly expressed during ontogenesis and spermatogenesis. Genetics and Molecular Research (2011) ,10(4):3963-3975. 

[20] YaNan Gong, WeiWei Li, JiangLing Sun, Fei Ren, Lin He, Hui Jiang, Qun Wang. Molecular cloning and tissue expression of the fatty acid-binding protein (Es-FABP) gene in female Chinese mitten crab (Eriocheir sinensis).BMC Molecular biology‚2010‚11:71. 

[21] Qian Mao, Xue li Hou, Ping Xiao, Xian ping Ying, Lin He and Qun Wang, Identification of proteins of accessory sex gland from Eriocheir sinensis by two-dimensional electrophoresis and MALDI-TOF mass spectrometry. Invertebrate Reproduction and Development, 2009. 53(3): p. 145–153.

[22] Qun Wang, Ying Wang, Lili Chen, Lin He, Weiwei Li, Hui Jiang. Expression characteristics of the SUMOylation genes SUMO-1 and Ubc9in the developing testis and ovary of Chinese mitten crab, Eriocheir sinensis. GENE. 2012. 501(2):135-143. 

[23] Qun Wang, Lili Chen, Ying Wang, Weiwei Li, Lin He, Hui Jiang. Expression characteristics of two ubiquitin/ribosomal fusion protein genes in the developing testis‚ accessory gonad and ovary of Chinese mitten crab‚ Eriocheir sinensis. Molecular Biology Reports. 2012. 39:6683-6692.

[24] Xing-Kun Jin, Wei-Wei Li, Lin Cheng, Shuang Li, Xiao-Nv Guo, Ai-Qing Yu, Min-Hao Wu,Lin He, Qun Wang.Two novel short C-type lectin from Chinese mitten crab, Eriocheir sinensis,are induced in response to LPS challenged. Fish & Shellfish Immunology 33 (2012) 1149e1158.

[25] Juan Wang, Di-An Fang, Yang Wang, Yuan-Li Wang, Lin Cheng, Lin He,and Qun Wang. Cathepsin A protein from accessory sex gland of the Chinese mitten crab (Eriocheir sinensis) plays a key role in spermatophore digestion. Journal of Insect Physiology. 2013, ,59 (2013) 953–960. DOI: 10.1016/j.jinsphys.2013.07.0032013.

[26] Lin Cheng, Xing-Kun Jin, Wei-Wei Li , Shuang Li, Xiao-Nv Guo, Juan Wang, Ya-Nan Gong, Lin He, Qun Wang. Fatty acid binding proteins FABP9 and FABP10 participate in antibacterial responses in Chinese mitten crab, Eriocheir sinensis. PLoS ONE 01/2013; 8(1): e54053. dio: 10.1371/journal.pone.0054053.

[17] Hong-Dan Yang, Qing Li, Ai-Qing Yu, Lin He*, Qun Wang. Molecular characterization and sub-celluiar distribution of JNK and JIP4 protein kinases in spermatogenesis and acrosome reaction of the Chinese mitten crab Eriocheir sinensis. Journal of Crustacean Biology, 00(0),1-11,2016. DOI: 10.1163/1937240X-00002468.

[16] Yuan-Li Wang, Dan Li, Hong-Dan Yang, Lin He, Wen-Juan Sun, Ze-Lin Duan, Qun Wang. The E3 ubiquitin ligase CRL4 regulates proliferation and progression through meiosis in Chinesemitten crab Eriocheir sinensis, Biology of Reproduction. 2016, 94(3), pp 1-14, 2016 3.

[17] Hong-Dan Yang, Qing Li, Ai-Qing Yu, Lin He*, Qun Wang. Molecular characterization and sub-celluiar distribution of JNK and JIP4 protein kinases in spermatogenesis and acrosome reaction of the Chinese mitten crab Eriocheir sinensis. Journal of Crustacean Biology, 00(0),1-11,2016. DOI: 10.1163/1937240X-00002468.

[16] Yuan-Li Wang, Dan Li, Hong-Dan Yang, Lin He, Wen-Juan Sun, Ze-Lin Duan, Qun Wang. The E3 ubiquitin ligase CRL4 regulates proliferation and progression through meiosis in Chinesemitten crab Eriocheir sinensis, Biology of Reproduction. 2016, 94(3), pp 1-14, 2016 3.