张鲁嘉

2016-2018,  美国加州理工学院(Caltech)，生物科学系(BBE)，博士后，合作导师：Grant Jensen（HHMI）

2007-2014,  华东理工大学，生物化学与分子生物学，博士学位

2001-2004,  南京工业大学，生物化工，硕士学位

1997-2001,  南京工业大学，微生物制药，学士学位

2016-至今，纽约大学(上海), 理论与计算化学中心，教授(兼）

2016-至今，华东师范大学，化学与分子工程学院，教授

2004-2016，华东理工大学，生物工程学院，讲师

实验室主页：www.lujialab.org.cn

主要研究方向为功性生物大分子(包括酶、生物材料等)的结构机制解析、理性设计及应用开发。具体研究内容包括：基于结晶、冷冻电镜等手段的蛋白质结构及复合生物大分子体系构造研究；结合分子模拟和量化计算的功能机制认知、结合人工智能的生物大分子设计技术开发以及计算生物学软件开发；基于关键蛋白质元件设计与适配的合成生物学体系构建及其在催化反应、化学品合成、生物材料、食品科学等领域的应用等。作为负责人先后承担国家重点研发项目课题及子课题、国家“863”项目子课题；国家自然科学基金面上项目、重点项目子课题等国家重大科研项目多项。在国际上首次实现了多个重要工业酶分子结构及细菌分泌系统构造的结晶/冷冻电镜解析；建立了多个全新生物大分子动态机制模型及人工智能精准设计策略；开发了国际领先且具有自主知识产权的蛋白质(酶、蛋白材料等)设计软件多套，获国家软件著作权3项，并已被广泛使用；在PNAS等重要国际期刊发表SCI论文60余篇；开发的新型工业酶及生物合成技术在宁夏夏盛等企业实现应用，创造了巨大经济社会效益，并因此获省部级科技奖励一等奖3项，二等奖2项。担任Frontiersin Molecular Biosciences等杂志编委。

中国食品学会，酶制剂分会，理事

上海市生物工程学会青年委员会，委员

国家自然科学基金通讯评议专家

上海市人才及科技奖励评审专家

上海市政府采购评审专家

国际学术期刊编委：Frontiersin Molecular Biosciences

国际学术期刊审稿人：ACS Synthesis Biology，Plos One，BBA-Proteins and Proteomics，Chemical Biology & Drug Design，Journal of Bioscience and Bioengineering，Biotechnology and Bioprocess Engineering.

‍‍‍‍

1. 利用结晶、Xray衍射及冷冻电镜技术的蛋白质(及其复合物)结构解析。

2. 结合分子动力学模拟、量子化学和高性能计算的酶分子催化机制解析、理性设计及相关软件开发。

3. 基于生物催化与转换技术的生物反应工程、合成生物学研究及其在食品、医药、材料、能源等领域的应用。

1.  生物化学反应工程(含实验)，授课对象：硕士生/博士生，3学分

2.  化工基础(含实验)，授课对象：本科生，3学分（72学时）

主持的国家级科研项目：

1. 国家科技部，国家重点研发计划项目课题，2020YFA0908402，蛋白质元件活性功能的分子机理研究，2020-11至2025-10，课题经费：217万

2. 国家科技部，国家重点研发计划项目子课题，2019YFA0905200，人造蛋白质合成的细胞设计构建及应用，2020-01至2024-12，子课题经费：160万

3. 国家自然基金委，联合基金重点项目子课题，U1805235，福建特色柑橘类水果琯溪蜜柚有效成分的确定及增值利用途径探索，2019-01至2022-12， 子课题经费：104万

4. 国家博士后基金面上项目(一等)，59批，2016M590661，基于高专一性脂肪酶理性设计的特定结构功能脂定向加工，2016-07至2017-12，项目经费：8万

5. 国家自然基金委，国家自然基委-广东省政府联合基金（第二期），U1501501，“天河二号”超级计算机应用专项，2016-01至2017-12，项目经费97万（等值“天河二号”超级计算机机时形式资助）

6. 国家自然基金委，面上项目，31571786，基于高活性血管紧张素转化酶抑制寡肽代谢和构效机制分析的蛋白食品可控串联酶解新策略研究，2016-01至2019-12，项目经费：78.6万

7. 国家科技部，国家“863”计划项目子课题，2012AA020403，酶分子规模化改造计算设计关键技术研究及应用，2012-01至2015-12， 子课题经费：50万

主持的其他科研项目：

1. 宁夏夏盛实业有限公司，企业委托项目，蛋白酶等工业酶制剂的活性改造，2018-12至于2024-11，100万

2. 天罗诊断科技江苏有限公司，企业委托项目，核酸检测及诊断用酶底物谱拓展研究与应用，2020-01至2023-12，20万

3. 材料化学工程”国家重点实验室，开放课题（招标项目），KL19-12，高性能蛋白粘附材料的精确设计及高效表达，2020-01至2021-12，课题经费：8万

4. 生物反应器工程国家重点实验室，“生物反应器工程国家重点实验室”开放课题,，分枝杆菌甾醇C17位侧链C22位点降解机制研究，2015-06至2018-05, 课题经费：5万

5. 国家教育部，中央高校基本科研业务费专项资金资助，W1214057，基于高性能计算的脂肪酶虚拟底物筛选系统的设计与开发，2012-10至2014-09，项目经费：12万

6. 材料化学工程国家重点实验室，开放课题，KL10-09，脂肪酶催化生物质衍生物的反应机制研究，2010-01至2012-12，课题经费：5万

7. 上海华谊（集团）公司,华东理工大学研究中心技术研究项目，生物法生产S-腺苷甲硫氨酸，2007-01至2010-12，项目经费：8万

参加的国家级重大项目：

1.  国家自然基金委，重点项目、胰岛素降解酶在隐孢子虫入侵中的作用机制、2017/01-2021/12，280万

2. 国家科技部，国家“973”计划项目，2012CB721000，微生物药物合成相关的若干重要酶蛋白的构效关系与进化，2012-01至2016-10，2500万元

3. 国家科技部，“重大新药创制”科技重大专项项目，2009ZX09306-001，生物技术药物中试放大及分离纯化技术平台,2012-01至2015-12, 800万元

4. 国家教育部，中央高校交叉学科与重大项目培育基金，新型生物催化剂创制方法及其产业化应用技术，2011-01至2014-12，160万元 

5. 国家科技部，国家“973”计划项目，2009CB724700，新一代生物催化和生物转化的科学基础，2009-01至2013-08，2500万元

发表学术论文:

1.     Yue Zhao, Yulu Miao, Fengdong Zhi, Yue Pan, Jianguo Zhang, XuepengYang, John Z. H. Zhang*, Lujia Zhang^*. Rational Design ofPepsin for Enhanced Thermostability via Exploiting the Guide of StructuralWeakness Stability.Frontiers in Physics.2021, 9:258

2.     Lujia Zhang, Jinkai Zheng, Mingzhe Ma, YueZhao, Jia Song, Xin Chen, Wanxiu Cao, Xiao He, Changhu Xue, Qingjuan Tang*. Drug-guidedscreening for pancreatic lipase inhibitors in functional foods. Food &function.2021, 12 (10):4644-4653

3.     Meng Ma, Bei Gao, Yao Liu, Bohuan Fang, Min Jiang, Tong Zhu,Zenghui Zhang*, Lujia Zhang^*. Isolation and application ofaflatoxin-oxidase based on magnetic protein label.Shengwu Jiagong Guocheng.2021, 19 (1):1-7

4.     Yao Liu, Yalong Cong, Chuanxi Zhang, Bohuan Fang, Yue Pan, QiangziLi, Chun You, Bei Gao, John Z. H. Zhang, Tong Zhu*, Lujia Zhang^*.Engineering the biomimetic cofactors of NMNH for cytochrome P450 BM3 based onbinding conformation refinement. RSC Advances. 2021, 11 (20):12036-12042

5.     Mohammed Kaplan, Yuhang Wang, Georges Chreifi, Lujia Zhang, Yi-Wei Chang, Grant J.Jensen*. Programmed Flagellar Ejection in Caulobacter crescentus LeavesPL-subcomplexes. Journal of Molecular Biology. 2021, 433 (13):167004

6.     Yanfang Han, Liping He, Yifei Qi, Yue Zhao, Yue Pan, Bohuan Fang, ShaLi, John Z. H. Zhang*, Lujia Zhang*.Identification of three newcompounds that directly target human serine hydroxymethyltransferase 2. ChemicalBiology & Drug Design. 2021, 97 (2):221-230

7.     Yalong Cong, Yinghui Feng, Hui Ni, Fengdong Zhi, Yulu Miao, BohuanFang, Lujia Zhang*, John Z. H.Zhang*. Anchor-Locker Binding Mechanism of the Coronavirus Spike Protein toHuman ACE2: Insights from Computational Analysis. Journal of ChemicalInformation and Modeling. Journal of Chemical Information and Modeling. 2021,61(7):3529–3542

8.     Ruiyao Chen, Yulu Miao, Xuan Hao, Bei Gao, Mingzhe Ma, John Z. H.Zhang, Rui Wang, Sha Li, Xiao He*, LujiaZhang*. Investigation on the characteristics and mechanisms of ACEinhibitory peptides by a thorough analysis of all 8000 tripeptides via bindingfree energy calculation.Food Science & Nutrition.2021, 9 (6):2943-2953

9.     Xiaolin Li, Yalong Cong, Mingzhe Ma, Zhineng You, Bei Gao, JohnZeng Hui Zhang^*, Lujia Zhang^*. An EnergyOptimization Strategy Based on the Perfect Conformation of Prolyl Endopeptidasefor Improving Catalytic6 Efficiency. Journal of Agricultural and Food Chemistry. 2020,68(18): 5129–5137

10.   Ying Wang, Verma, Pragya, LujiaZhang, Yaqi Li, Zhonghua Liu, Truhlar Donald G.^*, Xiao He*.M06-SX screened-exchange density functional for chemistry and solid-statephysics. Proceedings of the National Academy of Sciences of the UnitedStates of America. 2020,117(5): 2294-2301

11.   Liping He, Jingxiao Bao, YunpengYang, Suzhen Dong, Lujia Zhang, Yifei Qi*, John Z. H. Zhang*. Study ofSHMT2 Inhibitors and Their Binding Mechanism by Computational Alanine Scanning.Journal of Chemical Information and Modeling. 2019,59(9):3871-3878

12.   Qilong Tang, Wenli Wang, LujiaZhang, Yuan Liu*. Cloning, purification and biochemical characterization ofrecombinant Cathepsin L from Takifugu rubripes and its role in taste formation.Journal of Food Measurement and Characterization. 2019,14(1):485-491

13.   Lingyun Zhang, Dajiang Mei*,Yuanwang Wu, Chenfei Shen, Wenxin Hu, Lujia Zhang, Jinjin Li, YuandongWu, Xiao He. Syntheses,structures, optical properties, and electronic structures of Ba6Cu2GSn4S16 (G =Fe, Ni) and Sr6D2FeSn4S16 (D = Cu, Ag). Journal of Solid State Chemistry.2019,272:69–77

14.   Pujuan Zhang, Jinru Zhang, LujiaZhang, Jianan Sun, Yuan Li, Lian Wu, Jiahai Zhou, Changhu Xue, XiangzhaoMao*. Structure-based design of agarase AgWH50C from Agarivorans gilvus WH0801to enhance thermostability. Applied Microbiology and Biotechnology.2019,103(3):1289-1298

15.   Lijun Li, Zheyu Wu, Yue Yu, Lujia Zhang,Yanbing Zhu, Hui Ni*, Feng Chen. Developmentand characterization of an α-L-rhamnosidase mutant with improvedthermostability and a higher efficiency for debittering orange juice. FoodChemistry. 2018,245:1070-1078

16.   Bei Gao, Lei He, Dongzhi Wei, LujiaZhang*. Identification and magnetic immobilization of a pyrophilousaspartic protease from Antarctic psychrophilic fungus. Journal of FoodBiochemistry. 2018,42(6): e12691.

17.   Zhiqiang Yao, Shuiqin Jiang, Lujia Zhang*,Bei Gao, Xiao He, John Z. H. Zhang, Dongzhi Wei*. Crius: A novel fragment-basedalgorithm of de novo substrate prediction for enzymes. Protein Science. 2018,27:1526-1534

18.   Jingli Xie*, Xujun Chen, Junjie Wu, YanyanZhang, Yan Zhou, Lujia Zhang, YaJie Tang, Dongzhi Wei*. Antihypertensiveeffects, molecular docking study, and isothermal titration calorimetry assay ofangiotensin I‑converting enzyme inhibitory peptides from Chlorella vulgaris. Journalof Agricultural and Food Chemistry. 2018,66:1359-1368

19.   Bei Gao, Shuiqin Jiang, Liuzhu Wang,Lujia Zhang* , Dongzhi Wei* . Energy and conformation determine theenantioselectivity of enzyme. Biochemical Engineering Journal.2018,129:106-112

20.   Lei He, Youzhi Mao, Lujia Zhang,Hualei Wang, Siti Aisyah Alias, Bei Gao*, Dongzhi Wei. Functional expression ofa novel α-amylase from Antarctic psychrotolerant fungus for baking industry andits magnetic immobilization. BMC Biotechnology. 2017:17(22):1-13

21.   Min Jiang, Lujia Zhang, FengqingWang, Jie Zhang, Guosong Liu, Bei Gao*, Dongzhi Wei* . Novel application ofmagnetic protein: convenient one-step purification and immobilization ofproteins. Scientific Reports. 2017,7(1):13229

22.   Yunxiao Liang^#, Xiaoqing Ma^#,Lujia Zhang, Fuli Li, Zhen Liu, Xiangzhao Mao*. BiochemicalCharacterization and substrate degradation mode of a novel exotype β-agarasefrom Agarivorans gilvus WH0801. Journal of Agricultural and Food Chemistry.2017,65:7982-7988

23.   Mingrong Guo, Xujun Chen, Yanling Wu, LujiaZhang, Weixue Huang, Ying Yuan, Ming Fang, Jingli Xie*, Dongzhi Wei*.Angiotensin I-converting enzyme inhibitory peptides from Sipuncula(Phascolosoma esculenta): purification, identification, molecular docking andantihypertensive effects on spontaneously hypertensive rats. ProcessBiochemistry. 2017,63:84-95

24.   Ying Wang, Jinfeng Liu, Lujia Zhang,Xiao He*, John Z.H. Zhang*. Computational search for aflatoxin bindingproteins. Chemical Physics Letters. 2017,685:1-8

25.   Shuiqin Jiang, Lujia Zhang*, Zhiqiang Yao, Bei Gao, Hualei Wang,Xiangzhao Mao, Dongzhi Wei*. Switching a nitrilase from Syechocystis sp.PCC6803 to a nitrile hydratase by rationally regulating reaction pathways. CatalysisScience & Technology.2017,7(5):1122-1128

26.   Xiangzhao Mao*,Qianqian Liu, Yongqian Qiu, Xiaoqin Fan, Qingqing Han, Yanjun Liu, LujiaZhang, Changhu Xue*. Identification of anovel phospholipase D with high transphosphatidylation activity and itsapplication in synthesis of phosphatidylserine and DHA-phosphatidylserine. Journalof Biotechnology.2017,249:51-58

27.   LeiCheng, Haizhen Wu*, JingFei, Lujia Zhang, JiangYe, Huizhan Zhang. Preparation of monoclonal antibody against 4-amylphenol andhomology modeling of its Fv fragment. Chinese journal of cellular andmolecular immunology. 2017, 33(3):390-396.

28.   Shuai You, Tao Tu, Lujia Zhang, YuanWang, Huoqing Huang, Rui Ma, Pengjun Shi, Yingguo Bai, Xiaoyun Su, Zhemin Lin,Huiying Luo*, Bin Yao*. Improvement of the thermostability and catalyticefficiency of a highly active β-glucanase from Talaromyces leycettanus JCM12802by optimizing residual charge–charge interactions. Biotechnology forBiofuels. 2016,9:124 

29.   Xiaqin Yu^#, Lujia Zhang#,Xiaodan Miao, Yanyu Li, Yuan Liu*. The structure features of umami hexapeptidesfor the T1R1/T1R3 receptor. Food Chemistry. 2016,221:599-605

30.   Shuiqin Jiang, LujiaZhang*,DongbinCui, ZhiqiangYao, BeiGao, Jinping Lin, DongzhiWei*. The important roleof halogen bond in substrate selectivity of enzymatic catalysis. ScientificReports. 2016,6:34750

31.   Ying Yang, Jiaqi Sun, Junjie Wu,Lujia Zhang, Lei Du,Shingo Matsukawa, Jingli Xie*, Dongzhi Wei. Characterization of a Novel α- L -arabinofuranosidasefrom ruminococcus albus 7 and rational design for its thermostability. Journalof Agricultural andFood Chemistry.

32.   Zhiqiang Yao, Lujia Zhang*,Bei Gao, Dongbing Cui, Fengqing Wang, Xiao He,John Z. H. Zhang, Dongzhi Wei*. A semi-automated structure-based method topredict substrate of enzyme via molecular docking: a case study with Candidaantarctica Lipase B. Journal of Chemical Information and Modeling. 2016,56:1979-1994

33.   Ying Wang, Jinfeng Liu, TongZhu, Lujia Zhang, Xiao He*, John Z.H. Zhang*. Predicted PAR1 inhibitors from multiple computational methods. ChemicalPhysics Letters. 2016,659:295-303

34.   Xiao Liu, Jinfeng Liu, Tong Zhu,Lujia Zhang, Xiao He*, John Z. H. Zhang*. PBSA_E: a PBSA-based free energy estimator for protein-ligandbinding affinity. Journal of Chemical Information and Modeling. 2016,56:854-861

35.   Chuan Zhang, Lujia Zhang*, YangZhang, He Huang, Yi Hu *. Study on the stability and enzymatic propertyimprovement of porcine pancreas Lipase modified by ionic liquids usingmolecular simulation. Acta Chimicasinica. 2016,74(1):74-80

36.   Hualei Wang, Wenyuan Gao, HuihuiSun, Lifeng Chen, Lujia Zhang, Xuedong Wang*, Dongzhi Wei*. Proteinengineering of a nitrilase from Burkholderia cenocepacia J2315 for efficientand enantioselective production of (R)-o-chloromandelic acid. Applied andEnvironmental Microbiology. 2015,81(24):8469-8477

37.   Tao Tu, Huiying Luo, Kun Meng, Yanli Cheng,Rui Ma, Pengjun Shi, Huoqing Huang, Yingguo Bai, Yaru Wang, Lujia Zhang*,Bin Yao*. Improvement in thermostability of an Achaetomium sp. strain Xz8endopolygalacturonase via the optimization of charge-charge interactions. Appliedand Environmental  Microbiology. 2015,81(19): 6938-6944 

38.   Tao Tu, Kun Meng, Huiying Luo, Ossi Turunen,Lujia Zhang, Yanli Cheng, Xiaoyun Su, Rui Ma, Pengjun Shi, Yaru Wang,Peilong Yang, Bin Yao*. New insights into the role of T3 loop in determiningcatalytic efficiency of GH28 endo-polygalacturonases. Plos One. 2015,10(9) :1-16

39.   Bei Gao, Youzhi Mao, Lujia Zhang*,Lei He, Dongzhi Wei. A novel saccharifying a-amylase of Antarcticpsychrotolerant fungi Geomyces pannorum: gene cloning, functional expression,and characterization. Starch-Starke. 2015,68

40.  YouzhiMao, Yanchen Yin, Lujia Zhang, Siti Aisyah Alias, BeiGao*,DongzhiWei. Development of a novel Aspergillus uracildeficient expression system and its application in expressing a cold-adaptedα-amylase gene from Antarctic fungi Geomyces pannorum. Process Biochemistry.2015,50(10): 1581-1590

41.   Ying Yang, Lujia Zhang, Mingrong Guo,Jiaqi Sun, Shingo Matsukawa, Jingli Xie*, Dongzhi Wei. Novelα-L-Arabinofuranosidase from Cellulomonas fimi ATCC 484 and its substrate-specificity analysis with the aid of computer. Journal of Agricultural andFood Chemistry. 2015,63:3725-3733

42.   Dongbing Cui, Lujia Zhang*, ShuiqinJiang, Zhiqiang Yao, Bei Gao, Jinping Lin, Y. Adam Yuan, Dongzhi Wei*. Acomputational strategy for altering an enzyme in its cofactor preference toNAD(H) and/or NADP(H). Febs Journal. 2015,282(12): 2339-2351

43.   Zhenggui He, Lujia Zhang, Youzhi Mao,Jingchao Gu, Qi Pan, Sixing Zhou, Bei Gao*, Dongzhi Wei*. Cloning of a novelthermostable glucoamylase from thermophilic fungus Rhizomucor pusillus andhigh-level co-expression with α-amylase in Pichia pastoris. BMC Biotechnol.2014,14(1): 114 

44.  Bo Yin, Dongbing Cui, Lujia Zhang, Shuiqin Jiang, SatoruMachida, Y. Adam Yuan*, Dongzhi Wei*. Structural insights into substrate andcoenzyme preference by SDR family protein Gox2253 fromGluconobateroxydans. Proteins. 2014,82:2925-2935 

45.  Xu Liu,Chao Wang, Lujia Zhang, Zhiqiang Yao, Dongbing Cui, Liang Wu, JinpingLin*, YuRen Adam Yuan*, Dongzhi Wei. Structural and mutational studies on analdo-keto reductase AKR5C3 from Gluconobacter oxydans. Protein science. 2014,23: 1540-1549 

46.  LujiaZhang#, Bo Yin#, Chao Wang, Shuiqin Jiang, HualeiWang, Y. Adam Yuan*, Dongzhi Wei*. Structural insights into enzymatic activityand substrate specificity determination by a single amino acid in nitrilasefrom Syechocystis sp. PCC6803. Journal of Structural Biology. 2014,188(2): 93-101

47.  YalanLiu, Lujia Zhang, Mingrong Guo, Hongxi Wu, Jingli Xie*, Dongzhi Wei.Virtual screening for angiotensin I-converting enzyme inhibitory peptides fromPhascolosoma esculenta. Bioresources and Bioprocessing. 2014,1:17

48.  Peng Li,Jia Jia, Ming Fang, Lujia Zhang, Mingrong Guo, Jingli Xie*, Yuelan Xia,Li Zhou, Dongzhi Wei. In vitro and in vivo ACE inhibitory of pistachiohydrolysates and insilico mechanism of identified peptide binding withACE. Process Biochem.2014:49: 898-904

49.   Lujia Zhang, Bei Gao, Zuanning Yuan, XiaoHe, Y. Adam Yuan, John Z.H. Zhang*, Dongzhi Wei*. Structure,mechanism, and enantioselectivity shifting of lipase LipK107 with a simple way.BBA-proteins and Proteomics, 2014,1844:1183-1192

50.  Lujia Zhang, Xiaomang Tang, Dongbing Cui, Zhiqiang Yao, Bei Gao, ShuiqinJiang, Bo Yin, Y. Adam Yuan*, Dongzhi Wei*. A method to rationally increase protein stability based on thecharge-charge interaction, with application to lipase LipK107. Proteinscience. 2014,23: 110-116

51.  Dongbing Cui, Lujiang Zhang*, Zhiqiang Yao, Xu Liu, JinpingLin, Y. Adam Yuan, Dongzhi Wei*. Computational design of short-chaindehydrogenase Gox2181 for altered coenzyme specificity. Journal ofBiotechnology. 2013,167(4):386-392

52.  Jinliang Zhang, Sha Li, Hong Xu*, Peng Zhou, Lujia Zhang,Pingkai Ouyang. Purification of xylitol dehydrogenase and improved productionof xylitol by increasing XDH activity and NADH supply in Gluconobacter oxydans.Journal of Agricultural and Food Chemistry.2013,61: 2861-2867

53.  Chun Zhang, Kai Fan, Weitao Zhang, Ruixin Zhu, Lujia Zhang,Dongzhi Wei*. Structure-based characterization of canine-human chimericuricases and its evolutionary implications. Biochimie. 2012,94:1412-1420

54.  Huiting Song, Lichao Zhou, Lujia Zhang, Bei Gao, DongzhiWei, Yaling Shen, Rui Wang, Catherine Madzak, Zhengbing Jiang*. Construction ofa whole-cell catalyst displaying a fungal lipase for effective treatment ofoily wastewaters. Journal of MolecularCatalysis B-enzymatic.2011,71:166-170

55.  Bei Gao, Tao Xu, Jinping Lin, Lujia Zhang, Erzheng Su,Zhengbing Jiang, Dongzhi Wei*. Improving the catalytic activity of lipaseLipK107 from Proteus sp. by site-directed mutagenesis in the liddomain based on computer simulation. Journal of Molecular Catalysis B-enzymatic.2011,68:286-291

56.   Tao Xu, Bei Gao, Lujia Zhang,Jingpin Lin, Xuedong Wang, Dongzhi Wei*. Template-based modeling of apsychrophilic lipase: conformational changes, novel structural features and itsapplication in predicting the enantioselectivity of lipase catalyzedtransesterification of secondary alcohols. Biochimica ET Biophysica ACTA-Proteins and Proteomics.2010,1804:2183-2190

57.  Tao Xu, Lujia Zhang, Xuedong Wang, Dongzhi Wei*. A novel protocol of energyoptimisation for predicted protein structures built by homology modelling. Molecular Simulation. 2010,36(13):1104-1109

58.  Tao Xu, Lujia Zhang, Erzheng Su, Dongbing Cui, XuedongWang*, Dongzhi Wei*. Disparity in productive binding mode of the slow-reactingenantiomer determines the novel catalytic behavior of Candida antarcticalipase B.Journal ofMolecular Catalysis B-enzymatic.2010,62:288-296

59.   Tao Xu, Lujia Zhang,Xuedong Wang*, Dongzhi Wei*, Tianbi Li. Structure-based substrate screening foran enzyme.BMC Bioinformatics. 2009,10(257):1-7

60.  Lujia Zhang, Tao Xu, Peiqing Yuan, Dongzhi Wei*. Optimizing strategiesresearch on the later stage of protein structure model. Chemical Journal ofChinese Universities-Chinese. 2008,29(5):977-980

61.  Qun Wu, Hong Xu*, LujiaZhang, Jun Yao, Pingkai Ouyang. Production, purification and properties ofγ-glutamyltranspeptidase from a newly isolated Bacillus subtilis NX-2. Journal of Molecular Catalysis B-enzymatic.2006,43(1-4):113-117

授权专利及软件著作权：

1. 一种酶固定化方法及其应用. 2020-11-27, 中国，ZL201710444356.8

2. 一种表达微管β亚基与蛋白A的D结构域融合蛋白的基因工程菌株及其构建方法，2020-11-3, 中国， ZL202010207523.9

3. 一种基于酵母二肽基肽酶III的抗体模拟物及其应用，2020-10-20，ZL202010192357.X

4.   一种基于α螺旋融合两种蛋白质且保持各自亚基活性的蛋白质融合设计方法, 2020-10-20,ZL202010192367.3

5. 一种具有抗氧化活性的大菱鲆鱼皮发酵产物. 2016-06-01,中国，ZL201610390378.

6. 一种天冬氨酸类蛋白酶及其编码基因与应用. 2016-01, 中国，ZL 201610052686.8

7. 一种α-淀粉酶及其编码基因与应用. 2016-01,中国，ZL 201610052870.2

8. 一种尼克酸生物学制备方法、相关的菌株及其腈水解酶诱导方法. 2010-12，中国，ZL 200910050127.3

9. 蛋白质热稳定计算设计系统（ETSS），V1.0，国家软件著作权登记号：    2013SR108451，2013年，编写语言：C++. 

10. 酶底物虚拟筛选系统（CAESVSS），V1.0，国家软件著作权登记号：2013SR108447，2013年， 编写语言：C++&python.

学术会议报告：

1.  基于酶分子动态行为特征认知的理性设计策略研究，特邀报告，第十届国际分子模拟与人工智能应用学术会议；中国，苏州，2021-5-29至2021-5-3

2. Regulation of primary and secondary reactions of nitrilase, 特邀报告，第二届国际合成生物学论坛,中国，南京，2019-9-24至2019-9-26

3. 蛋白酶酶切位点选择性机制解析及理性设计，会议报告，第十二届中国酶工程学术研讨会，中国，长春，2019.8.8-8.11

4. 腈水解酶空间选择性机制认知及理性设计, 特邀报告, 第八届国际分子模拟与信息技术应用学术会议,中国, 大连, 2016.9.24-9.26

5. 酶底物虚拟筛选与生长软件开发, 会议报告， 第十二届全国酶学学术讨论会,会议报告,中国, 威海, 2015.9.18-9.20

6. 酶的底物谱预测新技术, 会议报告，2015年中国酶工程与糖生物工程学术研讨会,“诺维信青年优秀报告”奖, 中国, 镇江, 2015.8.21-8.23

7. 酶分子的理性设计策略研究及应用软件开发, 特邀报告, 第七届国际分子模拟与信息技术应用学术会议,中国, 苏州, 2014.10.26-10.28

8. 在结构与功能间搭桥, 特邀报告, 第六届国际分子模拟与信息技术应用学术会议, 中国, 南京, 2012.5.13-5.15

本人获得的省部级一等学术奖励：

1. 2016年，植物源生物活性物质精提用酶制剂的研发与应用. 中国商业联合会, 中国商业联合会科学技术奖全国商业科技进步奖, 一等奖

2. 2012年，手性非天然氨基酸生物催化制备技术及其应用，上海市人民政府，上海市科技进步奖，一等奖

3. 2010年，工业酶制剂的创制与产业化关键技术. 上海市人民政府，上海市科技进步奖，一等奖

本人获得的省部级二等学术奖励：

4. 2017年，生物酶理性改造与高效发酵关键技术及应用. 湖北省人民政府, 科技进步奖，二等奖

5. 2016年，海洋食品的生物制造关键技术与应用，国家海洋局（中国海洋工程咨询协会），国家海洋工程科学技术奖，二等奖

指导学生获得的教学奖励：

1. 2021年，第二届全国大学生化学实验创新设计大赛“微瑞杯”华东赛区，二等奖