宋高洁

2006-2009      中科院生物物理研究所结构与分子生物学中心      理学博士

2003-2006      北京师范大学生物化学与分子生物学系            理学硕士

1999-2003      安徽医科大学基础医学院                        理学学士

2018-至今      华东师范大学生命科学学院                              研究员

2014-2017      上海科技大学iHuman研究所                        研究副教授

2009-2014       哈佛医学院生物化学与分子药理学系               博士后

 

 IUCrJ, Protein&Cell, Cell reports, Science Advances等杂志审稿人

长期从事细胞表面受体的结构生物学研究，特别是G蛋白偶联受体（GPCR）的结构与信号传导机制研究， 相关研究成果发表在Nature， Nature communication, PNAS， Faseb J等国际知名学术期刊。GPCR是一类重要的药物靶点受体，超过30%的上市药物作用在这类膜蛋白上。实验室综合利用生物化学及生物物理学方法（如X射线晶体学、冷冻电镜）来研究该类受体的结构与信号传导机理，同时也感兴趣其他重要药靶蛋白的结构与功能研究。实验室已建立起成熟的膜蛋白及可溶蛋白表达体系、以及纯化和结晶平台，并可依托上海同步辐射光源开展数据收集。

主讲或参与《生物化学》《结构生物学进展》《现代生物学技术原理与应用》等课程

实验室主持或参与多项国家自然科学基金、 国家重点研发计划、以及上海市基础科学研究项目等。

1.   Fu C^#, Huang W^#, Tang Q^#, Niu M, Guo S, Langenhan T, Song G*, Yan J*. Unveiling Mechanical Activation: GAIN Domain Unfolding and Dissociation in Adhesion GPCRs. Nano Lett. 2023 Oct 13. doi: 10.1021/acs.nanolett.3c01163. Epub ahead of print. PMID: 37831892.

 2.   Zhang S#, Wang F#, Zhang D#, Liu D, Ding W, Springer TA, Song G*. Structural insights into MIC2 recognition by MIC2-associated protein in Toxoplasma gondii. Commun Biol. 2023 Aug 31;6(1):895. doi: 10.1038/s42003-023-05277-0. PMID: 37652989; PMCID: PMC10471735.

3.   Wang F, Wang Y, Qiu W, Zhang Q, Yang H, Song G*. Crystal Structure of the Extracellular Domains of GPR110. J Mol Biol. 2023 Jan 28;435(6):167979. doi: 10.1016/j.jmb.2023.167979. Epub ahead of print. PMID: 36716818. 

4.   Chen Y^#, Zhang J^#, Weng Y^#, Xu Y, Lu W, Liu W, Liu M, Hua T*, Song G*, (2022) Cryo-EM structure of the human adenosine A2B receptor-Gs signaling complex. Sci. Adv.8, eadd3709 23 December 2022

5.   Chen L, Zhang S, Xue N, Hong M, Zhang X, Zhang D, Yang J, Bai S, Huang Y, Meng H, Wu H, Luan C, Zhu B, Ru G, Gao H, Zhong L, Liu M, Liu M, Cheng Y, Yi C, Wang L, Zhao Y*, Song G*, Li D*. (2022) Engineering a precise adenine base editor with minimal bystander editing. Nat Chem Biol. 2022 Oct 13. doi: 10.1038/s41589-022-01163-8. Epub ahead of print. PMID: 36229683.

6.   Cui M, Zhou Q, Xu Y, Weng Y, Yao D, Zhao S, Song G*. Crystal structure of a constitutive active mutant of adenosine A2A  receptor. IUCrJ. 2022 Mar 17;9(Pt 3):333-341. doi: 10.1107/S2052252522001907. 

7.   Niu M, Xu S, Yang J, Yao D, Li N, Yan J, Zhong G, Song G.*(2021) Structural basis for CD97 recognition of the decay-accelerating factor CD55 suggests mechanosensitive activation of adhesion GPCRs. J Biol Chem. May 13:100776. doi: 10.1016/j.jbc.2021.100776. 

8.   Wu, F., Yang, L., Hang, K., Laursen, M., Wu, L., Han, G.W., Ren, Q., Roed, N.K., Lin, G., Hanson, M.A., Jiang, H.L., Wang, M.W., Reedtz-Runge, S., Song, G.*, Stevens, R.C.* (2020) Full length human GLP1 receptor structure without orthosteric ligands. Nature Communications.11:1272. Doi: 10.1038/s41467-020-14934-5c

9.  Xu, Y., Wang, Y., Wang, Y., Liu, K., Peng, Y., Yao, D., Tao, H., Liu, H. & Song, G.*(2019) Mutagenesis facilitated crystallization of GLP-1R. IUCrJ. Oct 17;6(Pt 6):996-1006. 

10.  Pu M*, Xu Z*, Peng Y*, Hou Y, Liu D, Wang Y, Liu H^#, Song G^#, and Liu ZJ^# (2017) Protein crystal quality oriented disulfide bond engineering. Protein & Cell. 2017 Oct 16. doi: 10.1007/s13238-017-0482-7 

11.  Yang L, Liu X, Yang Y, Tang Y, Zhang M, Tan M, Cheng S, Xu Y, Yang L, Yang H, Song G^# and Huang W^# (2017) Biochemical features of the adhesion G protein-coupled receptor CD97 for its functions of auto-proteolysis and HeLa cell attachment. Acta Pharmacologica Sinica. Jan;38(1):56-68. doi: 10.1038/aps.2016.89

12.   Song G, Yang D, Wang Y, Graaf C.D, Zhou Q, Jiang S, Liu K, Cai X, Dai A, Lin G, Liu D, Wu F, Wu Y, Zhao S, Ye L, Han G.W, Lau J, Wu B, Hanson M.A, Liu Z.J^#, Wang M.W^#, and Steven R.C^# (2017) Human GLP-1 receptor transmembrane domain structure in complex with allosteric modulators. Nature. 2017 May 17. doi: 10.1038/nature22378.

13.   Zhang H*, Qiao A*, Yang D*, Yang L*, Dai A, Graaf Cd, Reedtz-Runge S, Dharmarajan V, Zhang H, Han GW, Grant TD, Sierra R, Weierstall U, Nelson G, Liu W, Wu Y, Ma L, Cai X, Lin G, Geng Z, Dong Y, Song G, Griffin PR, Lau J, Cherezov V, Yang H, Hanson MA, Stevens RC, Jiang H, Wang MW, Zhao Q & Wu B (2017) Structure of the full-length glucagon class B G protein-coupled receptor. Nature. 2017 May 17. doi: 10.1038/nature22363.

14.  de Graaf C*, Song G*, Cao C*, Zhao Q, Wang MW, Wu B, Stevens RC^#. (2017) Extending the Structural View of Class B GPCRs. Trends Biochem Sci. 2017 Nov 10. doi: 10.1016/j.tibs.2017.10.003. 

15.  Song G and Springer TA. (2014) Structures of the Toxoplasma gliding motility adhesin. PNAS. 111(13):4862-7. doi: 10.1073/pnas.1403059111.

16.   Song G*, Cheng C*, Li Y, Shaw N, Xiao ZC, Liu ZJ. (2014) Crystal structure of N-terminal domain of human cytokine- induced apoptosis inhibitor anamorsin. Proteins. 82(6):1066-71. doi: 10.1002/prot.24443. (* equal contribution)

17.    Song G, Koksal AC, Lu C, Springer TA. (2012) Shape change in the receptor for gliding motility in plasmodium sporozoites. PNAS 109(52): 21420-25. doi: 10.1073/pnas.1218581109.

18.   Song G, Li Y, Cheng C, Zhao Y, Gao A, Zhang R, Joachimiak A, Shaw N, Liu ZJ. (2009) Structural insight into acute intermittent porphyria. FASEB J . 23(2): 396-404. doi: 10.1096/fj.08-115469.