管宁子

2010.09-2015.12：硕博连读，发酵工程，江南大学，师从陈坚、堵国成、刘龙教授研究团队

2006.09-2010.06: 本科，生物工程，江南大学，保送江南大学学术研究生

2018.10-：副研究员，生命科学学院，华东师范大学，叶海峰团队

2016.03-2018.01：博士后，Georgia Institute of Technology，Rachel Chen 团队

管宁子，华东师范大学生命科学学院副研究员，2016年在江南大学获得发酵工程博士学位，2016-2018年在美国佐治亚理工学院进行博士后研究工作，2018年起在华东师范大学工作。主要致力于代谢工程与微生物合成生物学在疾病治疗中的应用研究。其研究成果已经在ACS Synthetic Biology、Biotechnology & Bioengineering等国际学术期刊发表发表第一/通讯作者SCI论文十余篇。申请并授权国内外专利3项。获得国家自然科学基金青年基金，以学术骨干参与国家重点研究计划合成生物学专项。

研究方向包括代谢工程与微生物合成生物学在疾病治疗中的应用研究：

1. 益生菌传感器的开发与应用；

2. 光遗传学；

3. 肿瘤免疫治疗。

1. 2020-2022，国家自然科学基金青年项目“远红光调控基因表达工程细菌的设计、构建及应用研究”

2. 2020-2024，国家重点研发计划“人工基因回路设计、构建及其用于代谢疾病智能诊疗的研究”子课题二“基于基因回路精准调控的定制化细胞设计”

发表论文：

(1) Ningzi Guan^*, Long Liu, Microbial response to acid stress: mechanisms and applications, Applied Microbiology and Biotechnology, 2020, 104(1):51-65.

(2) Yuanhuan Yu, Xin Wu, Ningzi Guan, Jiawei Shao, Huiying Li, Yuxuan Chen, Yuan Ping, Dali Li, Haifeng Ye. Engineering a far-red light–activated split-Cas9 system for remote-controlled genome editing of internal organs and tumors. Science Advances, 2020. 6(28):eabb1777.

(3) Yidan Wang, Shuyong Liao, Ningzi Guan, Yuanxiao Liu, Kaili Dong, Wilfried Weber, Haifeng Ye. A versatile genetic control system in mammalian cells and mice responsive to clinically licensed sodium ferulate. Science Advances, 2020. 6(32):eabb9484.

(4) Ningzi Guan, Jianghua Li, Guocheng Du, Jian Chen, Long Liu^*, Microbial production of nutraceuticals: challenges and prospects. In book: Systems and Synthetic Biotechnology for Production of Nutraceuticals, 2019.

(5) Ningzi Guan, Hyun-Dong Shin, Lingfeng Long, Parastoo Azadi, Rachel Chen^*, TCA-Cycle powered synthesis of fucosylated oligosaccharides,Glycobiology, 2018, 28(7):468-473.

(6) Ningzi Guan, Juping Yu, Wei Liu, Rachel Chen^*,A high-yielding enzymatic process for production of bioactive β-1,3-glucan oligosaccharides with defined size,Journal of Glycobiology, 2018, 7:131.

(7) Ningzi Guan, Bin Du, Jianghua Li, Hyun-Dong Shin, Rachel Chen,Guocheng Du, Jian Chen, Long Liu^*, Comparative genomics and transcriptomics analysis-guided metabolic engineering of Propionibacterium acidipropionicifor improved propionic acid production. Biotechnology and Bioengineering, 2018, 115(2):483-494.

(8) Ningzi Guan, Rachel Chen^*, Recent technology development for the biosynthesis of Human Milk Oligosaccharide, Recent Patents on Biotechnology,2018, 12(2):92-100.

(9) Ningzi Guan, Jianghua Li, Hyun-Dong Shin, Guocheng Du, Jian Chen, Long Liu^*, Microbial response to environmental stresses: from fundamental mechanisms to practical applications, Applied Microbiology and Biotechnology, 2017, 101(10):3991-4008.

(10) Jing Wang, Le Yang^#, Xun Cui, Zhe Zhang, Lichun Dong^*, Ningzi Guan ^*, A DNA bubble-mediated gene regulation system based on thrombin-bound DNA aptamers, ACS Synthetic Biology, 2017, 6(5):758-765.

(11) Jing Wang, Xun Cui, Le Yang, Zhe Zhang, Liping Lv, Haoyuan Wang, Zhenmin Zhao, Ningzi Guan, Lichun Dong^*,Rachel Chen^*, A real-time control system of gene expression using ligand-bound nucleic acid aptamer for metabolic engineering, Metabolic Engineering, 2017, 42(7):85-97.

(12) Long Liu, Ningzi Guan, Jianghua Li, Guocheng Du^*, Jian Chen,Development of GRAS strains for nutraceutical production using systems and synthetic biology approaches: Advances and prospects, Critical Reviews in Biotechnology,2017, 37(2):139-150.

(13)  Ningzi Guan, Jianghua Li, Hyun-Dong Shin, Guocheng Du, Jian Chen, Long Liu^*, Metabolic engineering of acid resistance elements to improve acid resistance and propionic acid production of Propionibacterium jensenii, Biotechnology and Bioengineering, 2016, 113(6):1294-1304.

(14)  Long Liu^*, Ningzi Guan^#, Gexin Zhu, Jianghua Li, Hyun-Dong Shin, Guocheng Du, Jian Chen, Pathway engineering of Propionibacterium jensenii for improved production of propionic acid, Scientific Reports, 2016, 6:19963.

(15) Ningzi Guan, Jianghua Li, Hyun-Dong Shin, Jing Wu, Guocheng Du, Zhongping Shi^*, Long Liu^*,Jian Chen, Comparative metabolomics analysis of the key metabolic nodes in propionic acid synthesis in Propionibacterium acidipropionici, Metabolomics, 2015, 11(5):1106-1116.

(16) Ningzi Guan, Gexin Zhu, Jianghua Li, Hyun-Dong Shin,Jing Wu, Zhongping Shi^*, Long Liu^*, Engineering propionibacteria as versatile cell factories for the production of industrially important chemicals: advances, challenges, and prospects, Applied Microbiology and Biotechnology, 2015, 99(2):585-600.

(17) Ningzi Guan, Hyun-Dong Shin, Rachel Chen,Jianghua Li,Long Liu^*,Guocheng Du^*,Jian Chen,Understanding of how Propionibacterium acidipropionici respond to propionic acid stress at the level of proteomics, Scientific Reports,2014, 4:6951.

(18) Ningzi Guan, Long Liu^*,Hyun-Dong Shin, Rachel Chen,Juan Zhang, Jianghua Li,Guocheng Du, Zhongping Shi^*, Jian Chen, Systems-level understanding how Propionibacterium acidipropionici respond to propionic acid stress at the microenvironment levels: Mechanism and application, Journal of Biotechnology,2013, 167(1):56-63.

(19) Ningzi Guan, Long Liu, Gexin Zhu, Qiaoyan Xu, Jianghua Li, Guocheng Du, Jian Chen,Genome shuffling improves acid tolerance of Propionibacterium acidipropionici and propionic acid production, Advances in Chemistry Research, 2012, 15:143-52.

授权专利：

1.  Jian Chen, Guocheng Du, Long Liu, Jianghua Li, Ningzi Guan. Method for improving acid tolerance of Propionibacterium acidipropionici. Patent number: 14/047,968.

2.  刘龙，陈坚，堵国成，李江华，管宁子。一种提高产酸丙酸杆菌丙酸产量的方法。专利号：CN201410567748.X。

3.  刘龙，陈坚，堵国成，李江华，管宁子。一种耐酸的大肠杆菌工程菌。专利号：CN201410563738.9。